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KMID : 0903619870280030255
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Journal of the Korean Society for Horticultural Science
1987 Volume.28 No. 3 p.255 ~ p.268
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Effect of Ca , Mg , myo - Inositol and Pectin on Organogenesis of Saintpaulia ionantha Wendl . Cultured in vitro
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Abstract
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n order to attempt to reduce an occurrence of somatic mutation during the course of in vitro callus growth and organ differentiation of Saintpaulia cv. Delaware and cv. Julianne, varied levels of Ca, Mg, myo-inositol and pectin were supplemented to MS medium, and overall plant growth responses were investigated in relation to the following aspects;
1. A large quantity multiplication of the in vitro cultured tissue was attained with solid media, and the greater callus formation was noted in the liquid than the solid medium. The greatest of callus and shoot formation was obtained with solid media containing 880㎎/ℓ Ca and 100㎎/ℓ myoinositol.
2. The addition of myo-inositol also increased Ca content of the plant tissue in vitro derived, and thus an addition of increased level of myo-inositol to the medium always showed the greatest level of Ca in the tissue. An addition of more pectin to the medium also increased Ca level in the tissue, and Mg and K levels in the tissue were contrarily reduced as Ca level in the medium was increased. More Ca content was found in the calli than shoots usually. Mg content showed indifference between two different tissues, and almost doubled amount of K was accumulated in shoots when compared with calli.
3. When doubled level of Mg was supplemented to the solid medium in the standard level of myo-inositol-induced medium, the greatest callus growth and shoot formation were attained, and no shoot formation took place if no Mg contained in the medium.
4. The addition of increased level of Ca to the standard-pectin-level medium resulted in the greatest callus growth and shoot formation. An excess addition of Ca level to the pectin-treated medium markedly suppressed Mg and K absorptions in the tissue, and the addition of increased level of Ca to the increased-pectin level medium showed increased level of pectin in the tissue.
5. More chlorophyll content was observed in case of without supplementing any myo-inositol and Ca in the medium than with, and that was decreased as both the myo-inositol and Ca levels were increased. The appearance of chlorophyll content in Ca plus pectin treatments was much resembled to the aforementioned
6. The whole growth and the contents of Ca, Mg, K, pectin substance and chlorophyll in `Delaware´ were higher than that of `Julianne´, but there was no difference between two cultivars.
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